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Virulence characterization of Campylobacter jejuni isolated from resident wild birds in Tokachi area, Japan OAK
SHYAKA, Anselme; KUSUMOTO, Akiko; CHAISOWWONG, Warangkhana; OKOUCHI, Yoshiki; FUKUMOTO, Shinya; YOSHIMURA, Aya; KAWAMOTO, Keiko; 楠本, 晃子; 福本, 晋也; 川本, 恵子.
The prevalence of Campylobacter jejuni in wild birds is a potential hazard for human and animal health. The aim of this study was to establish the prevalence of C. jejuni in wild birds in Tokachi area, Hokkaido, Japan and investigate their virulence in vitro. In total, 173 cloacal swabs from individual wild birds were collected for the detection of Campylobacter spp. Thirty four samples (19.7%) were positive for Campylobacter of which 94.1% (32/34 samples) were C. jejuni. Additionally, one C. coli and one C. fetus were isolated. Seven C. jejuni isolates (one from crows and the other from pigeons) had important virulence genes including all three CDT genes (cdtA, cdtB and cdtC) and flaA, flaB, ciaB and cadF, and the other isolates were lacking cdtA gene....
Palavras-chave: Campylobacter; Japan; Virulence; Wild bird.
Ano: 2015 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4177
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サルモネラのVBNC状態におけるストレス応答関連シグマ因子RpoSの役割 OAK
楠本, 晃子; KUSUMOTO, AKIKO.
2009年度～2010年度科学研究費補助金研究活動スタート支援研究成果報告書21880005
Palavras-chave: VBNC; VNC; ストレス応答; シグマ因子; RpoS; サルモネラ.
Ano: 2011 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2998
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食品における赤痢菌検出法の感度の向上 OAK
門田, 修子; 楠本, 晃子; 牧野, 壮一; 川本, 恵子; MONDEN, Shuko; KUSUMOTO, Akiko; MAKINO, Sou-ichi; KAWAMOTO, Keiko.
細菌性赤痢は，近年食品媒介感染が危惧されており，赤痢菌の食品からの迅速かつ高感度な検出法確立は急務であ る.本研究では，厚生労働省より配布された参考試験法中，検出結果に影響を与えると思われた手順に改良を加え新規 試験法とし，検出系の感度や迅速性の向上を図った.結果，新規試験法は食品検体からの赤痢菌検出において，参考試験法よりも10倍高感度であった.また，菌検出までに要する時間がおよそ21時間以内と，参考試験法より約3.5時間 の短縮ができた.以上より，新規試験法は迅速微量検出法であり，食品からの赤痢菌検出に有用であると考えられる. Outbreaks of shigellosis have been associated with traveling to areas with poor hygiene. However，the number of foodborne cases is increasing in industrialized countries. Since the infectious dose of Shigella spp. is as low as 10 cells，a rapid and sensitive method of detecting Shigella spp. in food is required. The Ministry of Health，Labour and Welfare of ]apan (MHLW) released a detection protocol for Shigella spp. from oysters in 2002，but a number of problems with it， including low...
Palavras-chave: ジルコニアビーズ破砕法; リアルタイムPCR; Shigella sonnei; Real-time PCR; Shigella sonnei; Zirconia beads breakage procedure.
Ano: 2010 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2941
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